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Sep 26,  · The invention provides a method for increasing the stability and/or activity of a polypeptide at low pH and/or elevated temperatures. The invention further provides a method for increasing the melting temperature of a polypeptide.

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What is claimed is: An isolated polypeptide having a sequence selected from the group consisting of: An isolated polypeptide comprising at least about 10, at least about 20, at least about 30, at least about 50 at least about 60, at least about 70, at least about 80, at least about 90 or at least about consecutive amino acids from any of SEQ ID NOs: The isolated polypeptide of claim 1, wherein the variant has at least about

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The invention further provides a method for increasing the melting termperature of a polypeptide. The copyright owner has no objection to the facsimile reproduction by anyone of the patent document or the patent disclosure as it appears in the U. Patent and Trademark Office patent file or records, but otherwise reserves any and all copyright rights. The government has certain rights in the invention. The original color versions can be viewed in Perez- Jimenez et al. The contents of Perez- Jimenez et al.

The disclosures of these publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art as known to those skilled therein as of the date of the invention described herein. This has created an increasing need for enzymes that are stable at a wider range of temperatures and pH.

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